Infectivity of nuclear polyhedrosis virus in continuous cell cultures. III. Comparative neutralization of nonoccluded and occluded virus of nuclear polyhedrosis viruses.
نویسندگان
چکیده
منابع مشابه
Characterization of nuclear polyhedrosis virus DNAs.
The nuclear polyhedrosis virus DNAs characterized and compared in this study consist of the singly-enveloped nucleocapsids (SNPV) of Trichoplusia ni and the bundles of nucleocapsids common to a single envelope (MNPV) from Spodoptera frugiperda and Rachiplusia ou. The SNPV and MNPV DNAs are very similar in hydrodynamic properties and molecular weights. In addition, the NPV DNAs are similar in si...
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There have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. While there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. Three possible ways have...
متن کاملPlaque assay of nuclear polyhedrosis viruses in cell culture.
The nuclear polyhedrosis virus of Autographa californica has been titrated in Spodoptera frugiperda cells by the plaque method, using a solid overlay which does not require either the use of modified culture medium or expensive purified agarose or the addition of culture medium as a liquid layer above the solid agarose. This assay is more sensitive than that using a viscous methyl cellulose ove...
متن کاملNucleotide sequence of the polyhedrin gene region of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus: placement of the virus in lepidopteran nuclear polyhedrosis virus group II.
The polyhedrin gene (polh) of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus (HzSNPV) was identified and shown by sequence analysis of the EcoRI I genomic fragment to encode a 246 amino acid polypeptide that has greater than 80% sequence identity to known polyhedrins. It is preceded by an AT-rich region containing the conserved late promoter motif TAAG, which was identified as a...
متن کاملCross infectivity of baculovirus, Spilarctia obliqua nuclear polyhedrosis virus against mulberry pest, Porthesia xanthorrhoea Kollar.
Spilarctia obliqua nucleopolyhedrosis virus (SoNPV) has been found effective against S. obliqua (Walker) as well as Porthesia xanthorrhoea Kollar. LC50 concentration of S. obliqua SoNPV against S. obliqua and P. xanthorrhoea was 2.5 x 10(4) and 3.7 x 10(4) POBs/ml, respectively. LT50 of SoNPV against S. obliqua was 5.73 days, while it was 6.98 days for P. xanthorrhoea. Cross infectivity of SoNP...
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ژورنال
عنوان ژورنال: Japanese journal of applied entomology and zoology
سال: 1991
ISSN: 0021-4914,1347-6068
DOI: 10.1303/jjaez.35.31